Journal article
Hypoxic preconditioning enhances survival of human adipose-derived stem cells and conditions endothelial cells in vitro
SL Stubbs, STF Hsiao, HM Peshavariya, SY Lim, GJ Dusting, RJ Dilley
Stem Cells and Development | Mary Ann Liebert | Published : 2012
Abstract
To grow more robust cardiac tissue for implantation in vivo, strategies to improve survival of implanted stem cells are required. Here we report the protective effects of hypoxic preconditioning (HPC) and identify mechanisms for improving survival of adipose-derived stem cells (ASC) in vitro. Human ASC were preconditioned for 24 h with hypoxia and then exposed to simulated ischemia for a further 24 h. HPC significantly increased ASC viability, and reduced cell injury and apoptosis compared with non-preconditioned cells under ischemic conditions, as shown by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), lactate dehydrogenase-release, and caspase activity assays. Prec..
View full abstractRelated Projects (1)
Grants
Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
The authors would like to acknowledge support and Principal Research Fellowship (to G.J.D.) from the National Health and Medical Research Council of Australia (400303 and 509271) and the Australian Postgraduate Award (to S. S.). The O'Brien Institute acknowledges the support of JO and JR Wicking Trust and Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program. The authors also wish to acknowledge the technical advice of Caroline Taylor, Effie Keramidaris, and Catherine Chang.